New PDF release: Advances in Enzymology and Related Areas of Molecular

By Alton Meister

ISBN-10: 0470123141

ISBN-13: 9780470123140

ISBN-10: 0471310719

ISBN-13: 9780471310716

Protein Tyrosine Phosphatases: Mechanism of Catalysis and Substrate Specificity (Z.-Y.

Zhang & J. Dixon).

Heterologous Expression of Mammalian P450 Enzymes (M. Waterman).

Aspartate Transcarbamylase from Escherichia Coli: job and law (W. Lipscomb).

constitution and Mechanism of Bovine Lens Leucine Aminopeptidase (H. Kim & W. Lipscomb).


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Yeast contain endoplasmic reticulum (ER) and NADPH cytochrome P450 reductase making them an excellent system for expression of microsomal P450. The ER is not profuse in yeast so that the amount of P450 expressed may not be high; nevertheless, transformed yeast are easy to grow, and thus adequate quantities of microsomes for both enzymatic and spectral analysis of recombinant forms of P450 can be obtained. Activities of P450 in yeast microsomes can be optimized by addition of purified NADPH cytochrome P450 reductase to the isolated ER membranes (4).

93. ,Stover, D. , and Walsh, K. , J . B i d . , 268, 6835-6838 (1993). 94. Pallen, C. , Lai, D. S. , Chia, H. , and Tong, P. , Biochem. J . , 276, 315-323 (1991). PROTEIN TYKOSlNE PHOSPHATASES 35 95. , J . Org. , 53, 617-624 (1988). 96. Kitas. E. A.. Perich, J. , Wade, J. , John, R. , and Tregear, G. , 30, 6229-6232 ( 1989). 97. Lanzetta, P. , Alvarez, L. , Reinach, P. , and Candia, 0. , Anal. , 100, 95-97 (1979). 98. Madden, J. , Bird, M. , Man, Y.. , and Myles, D. , A n d . , I Y Y , 210-215 (1991).

Acud. Sci. USA, 88,5949-5953 (1991). 113. Ingebritsen, T. , J . Biol. , 264, 7754-7759 (1989). 114. Koch, C. , Moran, M. , Science. 252, 668-674 ( 199 1) . 115. Domchek, S. ,Auger, K. , Burke, T. R. , and Shoelson, S. , Biochemistuy, 31, 9865-9870 (1992). 116. , Case, R. , Domchek, S. , Chaudhuri, M.. Baker, J. , and Shoelson, S. , Biochemistry, 32, 3197-3202 (1993). 117. , Shoelson, S. , Chaudhuri, M.. Gish, G . , Haser, W. , King, F.. , Lechleider, R. , Neel, B. , 36 118. 119. 120. 121. 122. 123.

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Advances in Enzymology and Related Areas of Molecular Biology, Volume 68 by Alton Meister

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