By A H Rebar; et al
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Additional info for A Guide to Hematology in Dogs and Cats
19 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 20 Figure 3-10A Hemocytometer grid lines. Erythrocyte and leukocyte count. Red = zones to be counted under high power for erythrocytes. White = zones to be counted under low power for leukocytes. 1 mm above the grid so that there is a known volume of fluid over each grid area. Right: The arrow indicates the direction in which the count should be made. Triple ruling: Cells touching top and left center lines are counted (shaded cells for the first row).
Hypochromia and poikilocytosis are evident. Hypochromic RBCs have a large area of central pallor due to reduced hemoglobin content. The polychromatophilic RBC (arrow) in this field has a vacuolated or moth-eaten cytoplasm. Compare these cells with those in Figure 43 (100x). Figure 4-5 Canine blood. New methylene blue stain. Reticulocytes are visible as pale yellow cells with basophilic precipitates of RNA (100x). Figure 4-6 Canine blood, Howell Jolly body. Small basophilic round inclusion in the RBC cytoplasm (arrow) is a remnant of a nucleus (100x).
Limitations ® The main drawback to the use of external laboratories is time; results are not usually available for several hours or until the next day. – Can be an issue for time-sensitive cases or pre-anesthetic screening. – Fresh blood smears should be prepared in-clinic and sent with EDTA samples is hematology is performed at reference laboratories. – Check with your reference laboratory and confirm that blood smears are at least scanned by trained professionals. ® Hematology is species specific.
A Guide to Hematology in Dogs and Cats by A H Rebar; et al